For a glossary of terms used in this consolidated operating guide, refer to section 11 of the qiasymphony sp as user manual general description. Detailed instructions for the qiasymphony sp maintenance can be found in the qiagen qiasymphony sp manual. Qiasymphony spas general description 122017 9 1 safety information before using the qiasymphony sp as instruments, it is essential that you read this user manual carefully and pay particular attention to the safety information. Before using the qiasymphony sp, it is essential that you read the user manuals carefully and pay particular attention to the safety information. This flexible and reliable system, based on a readyto. Extraction of the dna from these samples using manual. May 2015 qiasymphony sp protocol sheet preanalytix. Qiasymphony operating software guides the user through setting up a. Completevideo qiagen qiasymphony spas dna preparation. After assay setup, sample lists can be exported to selected realtime pcr cyclers. Qiasymphony spas user manual operating the qiasymphony as. So far deparaffinization of the ffpe tissue sections is achieved by manual xyleneethanol pretreatment followed by proteinase k digestion.
Automated extraction of gdna from buffy coat samples. Evaluation of the qiasymphony sp workstation for magnetic. A weekly preventative maintenance schedule is established for the qiasymphony sp instrument. Before using the qiasymphony sp as instruments, it is essential that you read this user manual carefully and pay particular attention to the safety information.
In the present study, the automated qiasymphony and manual qiaamp extraction systems qiagen, valencia, ca were assessed using paired aliquots derived from clinical wholeblood specimens and an inhouse, realtime pcr assay. We have a total of 2 of these systems left, each one includes qiasymphony sp and as module as well as the stand. Documents document view page qiagen qiasymphony spas. Completevideo qiagen qiasymphony spas dna preparation system qia symphony spas video 3 7729. With the qiasymphony dsp dna mini kit, in combination with the qiasymphony generead dna ffpe treatment kit and qiasymphony sp, it is possible to fully automate dna extraction from up to 96 ffpe samples. As directly interfaces with the qiasymphony sp, delivering fast, highly reproducible pcr setup. Qiasymphony a new level in lab automation duration. Qiasymphony workflows samples assays independent operation the qiasymphony as is currently under development, please inquire for availability. The company feels that the adding of qiasymphony sp. Blood rna tubes according the manufacturers instructions. Manualslib has more than 27 qiagen manuals checkout popular qiagen categories laboratory equipment manuals.
To reduce manual handling steps and minimize the risk of sample contamination, samples processed on the qiasymphony sp can be transferred automatically to the qiasymphony as integrated operation. Introduction in largescale population studies it is common to collect samples from thousands of donors. Qiasymphony paxgene blood ccfdna kit handbook preanalytix. Qiagen qiasymphony spas dna preparation user manual this is the document view page.
The investigator advanced protocol has a longer heating step that enhances dna binding to magnetic particles, while the high efficiency protocol reduces the loss of purified dna from low elution volumes. The as module interfaces with qiasymphony sp and is operated via the same easytouse, intuitive software. Qiagen launches qiasymphony sp device outside the u. For extra flexibility, the qiasymphony sp and as can also be operated independently.
Oring test, uvscan and wiping down of conveyor base tray, liquid waste. Workflow optimization with the qiasymphony system adds. The qiasymphony was able to extract large batches without crosscontamination of samples. Extracted ccfdna is compatible with different assay applications qpcr, nextgen sequencing, arrays. Management at qiagen is encouraged by the adoption of genereader ngs system as a userfriendly solution for nextgeneration sequencing. For a glossary of terms used in this consolidated operating guide, refer to section 11 of the qiasymphony spas user manual general description. Qiasymphony spas operating the qiasymphony sp qiagen. Maintenance procedure for qiasympony sp instrument a weekly preventative maintenance schedule is established for the qiasymphony sp instrument. Qiasymphony as module not sold separately as module compatible with various pipetting formats rotorgene discs and 96well plates. A revised version of chapters 4 of this manual now appears in the. Recently we introduced an automated solution for the dna extraction from ffpe tissue using the qiasymphony sp instrument in combination with the qiasymphony dna mini kit.
Opposite to that, the ipc was correctly amplified with 6. A dedicated range of qiasymphony kits increase laboratory efficiency since just a few kits in combination with optimized protocols cover an extensive range of starting materials and applications. Qiasymphony as qiasymphony as extends the capabilities of qiasymphony sp to include highly reliable and reproducible automated assay setup. Before using the qiasymphony spas instruments, it is essential that you read the user manuals carefully and pay particular attention to the safety information. Print bookmark for use with the qiasymphony spas instruments software version 4. Evaluation of four automated protocols for extraction of.
Mott center 275 east hancock detroit, michigan 48201 fax. Laboratories already operating a qiasymphony sp can easily upgrade their system. Qiasymphony sp manuals and user guides for qiagen qiasymphony sp. Used qiagen qiasymphony spas dna preparation dna related. Qiagen qiasymphony sp operating manual pdf download. Automated pcr assay setup seamless integration with qiasymphony sp. Qiasymphony certal kits, in combination with the qiasymphony sp, enable fully automated purification of nucleic acids from samples generated during production and purification of cellculturebased biotechnologyderived products, biopharmaceutical protein drugs e. Detailed instructions for the qiasymphony sp maintenance can be found in the qiagen qiasymphony sp. Qiasymphony spas user manual operating the qiasymphony sp. Novel extraction protocols for the qiasymphony sp improve dna yields from forensic casework samples. Qiasymphony dsp dna mini kit 192, 937236 from qiagen.
We have 1 qiagen qiasymphony sp manual available for free pdf download. Used qiagen qiasymphony sp dna related for sale dotmed. Refer to the qiasymphony spas user manual operating the. Work lists and rack files may be imported to the instruments, enabling automatic run definition before samples are received in the laboratory.
Qia symphony sp, fully automated magneticparticle rna, dna and protein purification for up to 96 samples. Since manual pipetting steps are eliminated, the quality and efficiency of the assays can be ensured even for. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Comparison of qiasymphony automated and qiaamp manual. A dedicated range of qiasymphony kits increase laboratory efficiency since just a few kits in c. Qiagen qiasymphony spas dna preparation user manual. Processing challenging forensic casework samples with new. Mahajan4 march 24, 2010 1this research was partially supported by nsf grants dms9527124, dmi0534862, and dmi0522796, as well as texas atp grant 973604010. Descriptions of the tabs, tools, and buttons are listed in separate tables. The instructions and safety information in the user manual must be followed to ensure safe operation of the instruments and to maintain the instruments in a safe condition. Qiasymphony sp as user manual operating the qiasymphony as. For use with qiasymphony sp as instruments software version 5. Yields varied depending on the type and donor of the tissue and ranged from 10. En qiasymphony spas user manual general description.
For instance, after the manual dna extraction, the amplification of the internal pcr control ipc was totally inhibited when concentration of humic acid exceeded 0. View online or download qiagen qiasymphony sp operating manual. The qiasymphony spas instruments provide standardized communication interfaces. The detection limits using the qiasymphony and qiaamp systems were similar 270 and 560 copiesml, respectively. Qiasymphony paxgene blood rna kit handbook 032016 5 figure 1. Qiasymphony sp pure performance 012008 ordering information product contents cat. Qiasymphony technology combines the speed and efficiency of silicabased. Qiagen kit handbooks and user manuals are available at.
The qiasymphony sp can save you time by processing 196 samples in batches of 24 with sample volumes up to 2 ml. Completevideo qiagen qiasymphony spas dna preparation system qia symphony spas video 2 7729. Automated dna extraction using the qiasymphony platform. Dsp protocol was able to extract dna from 24 hemagenebuffy coat dna samples in approximately 2 hours with no user interaction beyond initial instrument setup. Completevideo qiagen qiasymphony sp as dna preparation system qia symphony spas video 2 7729.
Qiasymphony cabinet sp cabi net for the qiasymphony sp 9020244 qiasymphony cabinet as cabi net for the qiasymphony as 9020245 qiasymphony cabinet spas cabinet for the qiasymphony sp and qiasymphony as 9020246 for uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Automated dna extraction from ffpe tissue using a xylene. Our preliminary results further suggest that the dna recovery of this platform is similar to the one of our manual protocol used for routine stain analyses. In addition, the latest version of the qiasymphony as software enables str pcr setup. Compared to the manual dna extraction procedure, the qiasymphony offers several advantages in terms of reproducibility and reliability. We recommend using elution microtubes cl, racked provided for elution. For use with qiasymphony spas instruments software version 5. The qiasymphony sp performs as well or better than current manual extraction methods without introducing contamination. Use of this product signifies the agreement of any purchaser or user of the qiasymphony paxgene blood rna kit to the following terms. The qiasymphony as is a module that directly interfaces with qiasymphony sp. Using a manual plunger operated pipette, the lysate was transferred to 2ml sample tubes sarstedt, numbrecht, germany and loaded onto the qiasymphony sp qiagen. Qiasymphony spas user manual operating the qiasymphony.
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